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-acetyl spinoff. This result's consistent with the development or collapse from the tetrahedral intermediate as the speed limiting step within the catalytic system of NagA.

Internet site distinct mutants of NagA ended up manufactured as a way to ascertain the roles of distinct residues in substrate recognition and catalytic operate. The steel articles along with the kinetic parameters to the mutants of NagA constructed for this investigation are presented in Desk 4.

The precise function from the metal cofactor is of particular desire for NagA as the two crystal constructions readily available for the enzyme from E. coli

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m observed for the several metals follow the development: Zn = Co > Mn > Cd > Fe > Ni . The Fe that contains enzyme was not completely reconstituted with metallic and thus the kinetic constants can be much larger if the metal web site was thoroughly occupied. The values for k